Matrix method of laser fluorimetry of complex organic compounds in water
Victor V. Fadeev, Tatiana A. Dolenko, Denis V. Il'in, Pavel N. Litvinov and Andrei A. Meshkantsov
Abstract
A matrix method synthesizing methods of kinetic and
non-linear laser fluorimetry is presented in this paper. It is assumed that in
the case of a rational selection of the range for photon flux density of the
exciting radiation, and of the range of time for measurements of fluorescence
kinetics, it is possible to determine a substantial number of molecular
photo-physical parameters of complex organic compounds. This could be achieved
by solving the inverse problems with the elements of the suggested matrix as
input data. As a first step in the development of this approach, numerical modelling
of the direct and inverse problems has been performed using the model of a
two-fluorophore complex with intermolecular energy transfer.
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History
Submitted: 07 June 2003
Revised: 09 February 2004
Accepted: 13 February 2004
Citation
Fadeev V V, T A Dolenko, D V Il'in, P N Litvinov & A A Meshkantsov, 2004. Matrix method of laser fluorimetry of complex organic compounds in water. EARSeL eProceedings 3(2), 222-226
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ISSN 1729-3782
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