Vol. 6, No. 2, 47-57, 2007
Luca Fiorani, Roberta Fantoni, Antonia Lai, Antonio Palucci, Konstantin A. Semyanov, Maria Sighicelli, Valeria Spizzichino and Peter A. Tarasov
Abstract
A new laser flow cytometer, based on a laser source of polarized light irradiating microscopic particles,
has been developed. The unpolarized and linearly polarized scattered light, as well as the side emitted light in different spectral bands, are measured,
allowing the simultaneous and real-time determination of size, refractive index, light scattering depolarization and fluorescence of each particle.
Tests with aqueous samples of polystyrene spheres, fluorescent or non fluorescent, and phytoplankton cells demonstrate that the system is able to retrieve
size and refractive index and that the light scattering depolarization and fluorescence measurements allows the sorting of particles otherwise indistinguishable.
Submitted: 11 June 2007
Revised: 04 Oct 2007
Accepted: 04 Oct 2007
Published: 19 Oct 2007
Responsible editor: Rainer Reuter
Citation
Fiorani L, R Fantoni, A Lai, A Palucci, K A Semyanov, M Sighicelli, V Spizzichino & P A Tarasov, 2007.
First simultaneous determination of size, refractive index, light scattering depolarization and fluorescence of phytoplankton cells by laser scanning flow cytometry.
EARSeL eProceedings, 6(2): 47-57
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EARSeL European Association of Remote Sensing Laboratories, Strasbourg, France |
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BIS-Verlag |