Non-linear fluorimetry for diagnostics of the phytoplankton state
Dmitri V. Maslov, Victor V. Fadeev, Evgenij E. Ostroumov and Sergei A. Burikov
Abstract
In this paper a new approach
for phytoplankton diagnostics based on the non-linear fluorimetry method
(saturation fluorimetry method) is proposed. This approach can be realised both
in a sample probe mode and in a remote sensing mode. The procedure for the
determination of the non-saturated fluorescence parameter which is proportional
to concentration of chlorophyll a
molecules and the photophysical parameter A
has been elaborated. Parameter A
includes the excitation cross‑section of chlorophyll a molecules, the lifetime of the excited
chlorophyll a molecules taking
into account all processes of a deactivation of excitation except the
singlet-singlet annihilation, and the maximum rate of the singlet-singlet
annihilation.
Laboratory experiments with
the axenic culture of eurihaline Thalassiosira
weissflogii have shown that A is
strongly dependent on the functional state of phytoplankton. The state was
changed by treatment with herbicide DCMU and by actinic light
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History
Submitted: 12 June 2003
Revised: 11 May 2004
Accepted: 28 June 2004
Citation
Maslov D V, V V Fadeev, E E Ostroumov & S A Burikov, 2004. Non-linear fluorimetry for diagnostics of the phytoplankton state. EARSeL eProceedings 3(3), 306-315
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ISSN 1729-3782
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